HY 192 DNA RNA oligo synthesizer mo le gaosiga maualuga
Fa'amatalaga
| Fua fa'atusa fa'atasi | 5noml-5umol. |
| Taimi ta'amilosaga fa'aopoopo | e oʻo atu ile 6-8 minute |
| Ta'amilosaga fa'asologa (20mer) | 2-3 itula |
| Fa'avae fagu phosphoramidite | 8 seti |
| Fa'avae fagu fa'afefe | 7 seti |
| fagu fa'avae | 60/240/480ml fa'a sikulima gutu fa'afeso'ota'i fagu fa'afefe |
| Auxiliary reagent fagu | GL38 gutu fagu, faʻaoga mo fagu faʻafefe 4L lautele. |
| Reagent auala ave taavale | kasa puipuia i lalo o le mamafa ituaiga |
| Lafoa'i suavai otaota | omiga lelei |
| Fua fa'atasi | 99% |
| Ole maualuga ole umi | sili atu i le 120mer |
| Paoa sapalai | Vaega e tasi 220V. |
| Galulue vevela | 20C°± 5C° |
| Susū fa'atusa | i totonu ole 40%. |
| Tumau o le taotoga | E mafai ona galue masani ma fa'aauau. |
| Mataitu | LCD |
| Fa'amaonia | 1 Tausaga |
Fa'aaliga
1. O reagent ta'itasi o se auala tuto'atasi mai le fagu vai e teu ai i le koluma fa'asologa e aunoa ma isi alalaupapa e sopoia.
2. O le activator ma le Phosphoramide e faʻaopoopo faʻasolosolo i le taimi o le faʻaogaina o le faʻagasologa ma faʻapipiʻi muamua i luga o le koluma faʻapipiʻi e faʻatino ai le tali.
3. Ua faʻapipiʻiina i papatusi e lua ma le aofaʻi o 192 synthesis columns ma 12-20 base bottle ports.
4. I le faaopoopo atu i 4 faavae masani ma synthetic reagents auxiliary, o loo i ai foi 8 suiga faavae, lea e mafai ona synthesized pe a manaomia, e pei o le thiomodification, po o isi fesuiaiga fluorescent ma faʻailoga faʻalua TAQMAN suʻesuʻe, ma isi E sili atu i le 8 faʻavae faʻapitoa.
5. O loʻo i ai le faʻapipiʻiina otometi ma le faʻamalosia o faʻavae e aunoa ma le manaʻomia mo le faʻafefiloi muamua o Phosphoramidites.
6. I le faagasologa atoa o le faʻasologa, o loʻo i ai se aofaʻiga faʻapitoa i totonu o le potu faʻapipiʻi (o le aofaʻi o le kesi e mafai ona fetuʻunaʻi) O loʻo faʻatumu le kesi puipui e puipuia ai le ea mai le ulufale atu i totonu o le faʻaogaina o le faʻaogaina ma aʻafia ai le lelei o le synthesis.
7. Reagents ma Phosphoramide e mafai ona utaina i ni fagu se lua i le taimi e tasi, lea e mafai ona faʻaleleia lelei le taimi o le tuʻufaʻatasiga tasi e aunoa ma le suia soo o reagents.
8. Faʻapipiʻiina i le faʻamama faʻamalo valve, ma acetonitrile ma argon e mafai ona faʻaogaina e faʻafefe ai paipa ma alalaupapa e aloese ai mai le poloka o paipa ma alatoto e mafua mai i le faʻaogaina umi o meafaigaluega.
9. O meafaigaluega o loʻo i ai galuega o le siakiina o le tagata lava ia ma le puipuiga, faʻamalo, taofi ma faʻaauau.
10. Tulaga faigaluega ma mana'oga mo le saogalemu e ogatasi ma tulaga fa'a-Saina ma fa'ava-o-malo po'o tulafono faatonutonu.
