Mailei mole mole phosphoramidit ma reagents
Fa'amatalaga o oloa
I le ABI ma le MilliGen/PerSeptive DNA synthesis suʻega, e mafai e le pusa sieve ona taofia le vai i totonu o fagu nitrile ma activator i lalo ifo o le 10 ppm, e aunoa ma le popole e uiga i le valve poʻo le valve throttle ua vaevaeina i le sub-sieve.O le pefu po'o le lint i luga o le ato afifi ua poloka.E mafai ona faʻaaoga saʻo mo le faʻafefeteina o le 500 ml, 1L, 2 L ma isi fagu solvent, ma e mafai foʻi ona faʻapipiʻiina faʻamatalaga faʻapipiʻi lalo.
O lo'o fa'aalia i lalo le malosi o le fa'avaivaia o le 10 g molecular mailei i 500ml, 1L, 4L nitrile samples ma vai eseese.
Fa'atusatusa ile mailei mole mole mai fafo
| 500 ml 197 ppm ACN | |||||
| Taimi(h) | 0 | 24 | 48 | 72 | 96 |
| HonyaBio | 197 | 33 | 16.5 | 6.5 | 6 |
| Isi Atunu'u | 197 | 43 | 27 | 15 | 15 |
| 1 L 143 ppm ACN | |||||
| Taimi(h) | 0 | 24 | 48 | 72 | 96 |
| HonyaBio | 143 | 48 | 32 | 20 | 15 |
| Isi Atunu'u | 142 | 47 | 36 | 23 | 15 |
| 4 L 141 ppm ACN | |||||
| Taimi(h) | 0 | 24 | 48 | 72 | 96 |
| HonyaBio | 141 | 95 | 94 | 84 | 73 |
| Isi Atunu'u | 141 | 96 | 95 | 85 | 72 |
Fa'atonuga ma le fa'aoga
O le mailei mole mole o loʻo faʻapipiʻiina, ma e manaʻomia ona tatalaina e tusa lava pe faʻaaogaina, ma e tatau ona faʻamautinoa le faʻamaufaʻailoga o le fagu reagent aʻo faʻaaogaina.
2g sieve mo le 24 itula e mafai ona faʻaititia le 165 ppm vai i totonu ole 500 ml nitrile ile 105 ppm.
5 g sieve mo le 24 itula e mafai ona faʻaititia le 172 ppm vai i totonu ole 500 ml nitrile ile 58 ppm.
10 g sieve 24 h E mafai ona fa'aititia le 166 ppm vai ile 1 L nitrile ile 68 ppm.
20 g sub-sieve e mafai ona fa'aititia le 162 ppm vai i totonu ole 4 L nitrile ile 109 ppm mo le 24 itula.
Matou te fautuaina 2 g subsieve mo 50-250ml fagu reagent, 5g mo 250-500ml fagu reagent, 10g mo 500-1000ml fagu reagent, ma 20g mo 1000-2000ml fagu reagent.
Faamautinoaga
Ta'itasi sub-screen afifi e feagai ma le pulea lelei atoatoa i le faagasologa o le gaosiga.Ma e tatau mo tagata faʻatau ona siaki le afifiina aʻo leʻi faʻaaogaina:
Muamua, fa'amaonia o lo'o atoatoa le masini fa'apipi'i.So'o se vacuum leakage po'o le ea ulufale o le a fa'aitiitia ai le fa'atinoga o le oloa.
Lona lua, fa'aeteete pe a tatalaina le afifiina e puipuia ai le mata'itusi fa'apipi'i i lalo o le ata mai le valuina, ma siaki pe o le ata o lo'o I ai se liki.
Togafitiga pe a uma ona faʻaaogaina
E le oona pe afaina, o le a afaina i vailaʻau ma solvents latou te faʻafesoʻotaʻi ma e tatau ona lafoai e pei o otaota leaga pe a uma ona faʻaoga.
Fa'ata'ita'iga ma fa'aoga
Ua matou tuʻuina atu muamua taga faʻamama i lapopoa o le 2 g, 5 g, 10 g, ma le 20 g., Ma isi faʻamatalaga e mafai ona faʻatulagaina e tusa ai ma manaʻoga o tagata faʻatau.
O le mailei mole e talafeagai mo le faʻaalia umi i le nitrile, ma e mafai foi ona faʻaaogaina mo le acetic acid, ether, acetic ether, butyl acetate, ava malosi, isopropanol, methanol, butanol, Phenol, pyridine, hydrochloric acid, nitric acid, phosphoric acid. , sulfuric acid, methyl chloride, nitrogen methyl imidazole, ma isi.
Atonu e le talafeagai mo le faʻaalia umi i le tetrahydrofuran, toluene, methyl formamide (DMF), methyl methyl amide (DMAc), N-methylpyrrolidone (NMP) ma isi fofo.
